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By M.Pavithra & G.Srividhya Iyer


In accordance with the present cosmological theory, the universe will proceed to expand indefinitely. So, it must freeze in due course after reaching temperatures too cold to sustain life. This theory is commonly known as heat-death or the big freeze. Leaving aside this potentially displeasing synopsis, unexpectedly in the lifetime of 21st century freezing, in contrast, has played a vital role in hematopoietic cell autotransplants for disorders such as plasma cell myeloma and lymphomas. Before two decades the genetic resources were getting depleted due to the devastation by man. So it is of paramount importance to preserve many from elite, economically predominant and endangered species to make them available in future. The traditional techniques to preserve species have failed due to various reasons. A new methodology had to be discovered for preservation of material in the long run. Cryopreservation is the technique to keep the cells, tissues and other biological samples alive. The materials have to be kept in a deep freezing point at subzero temperatures for the storage process. The specimen is generally being processed at −196°C. At such ultra-low temperatures, all the biological functions of the cells are halted and the cells tend to die. Cryopreservation assists the cells to stay alive in the freezing and thawing process. The ice development inside the cells can disintegrate the cell membrane. This can be stopped by regulating the freezing rate and deliberately selecting the freezing medium. In this research paper, we will explore the obstacles with cryopreservation and the emerging developments that could upgrade these methods. The paper also discusses the need for adopting appropriate legal framework in India to deal with cryopreservation controversial issues.
Cryopreservation; cryoprotectants; cryoprotective agent; cryobiology; thawing; germplasm; slow freezing; vitrification; ART; sperm; oocyte; embryo.

TypeResearch Paper
InformationLex Humanitariae: Journal for a Change, Volume II issue IV, Pages 631-637
ISSN 2582-5216
Creative CommonsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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